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DeCS
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Descriptor English:
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Chromatin Immunoprecipitation
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Descriptor Spanish:
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Inmunoprecipitación de Cromatina
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Descriptor Portuguese:
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Imunoprecipitação da Cromatina
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Synonyms English:
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Immunoprecipitation, Chromatin
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Tree Number:
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E05.393.170
E05.478.605.160
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Definition English:
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A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. |
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History Note English:
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2005
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Allowable Qualifiers English:
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Record Number:
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38826
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Unique Identifier:
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D047369
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Occurrence in VHL:
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Similar:
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DeCS CID-10 LILACS
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